Temperature variation causes interesting changes in the relaxation characteristics and natural abundance (^15)N and (^13)C CP/MAS spectra. Residual dipolar coupling effects vary in their manifestation within the (^13)C CP/MAS spectra of the polymorphic systems studied and comparison with the literature yields important information regarding molecular conformation. Nitrogen 15 enrichment and operation at higher magnetic field have been applied to reduce these second order effects.

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In the absence of antibiotic selection the plasmid is stable in the host but is unstable in continuous fermentation. The instability was identified as segregational using restriction mapping and Southern blots. It is caused by the high levels of constitutive gene expression in the clone; as a result a population of faster growing P(^ ) segregants rise to predominance in the fermenter.

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